Composite

Part:BBa_K1148013:Design

Designed by: Cécile Quéré   Group: iGEM13_Bordeaux   (2013-10-12)


4CL::STS (resveratrol synthase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal BglII site found at 1125
    Illegal BamHI site found at 194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
    Illegal AgeI site found at 982
    Illegal AgeI site found at 1091
    Illegal AgeI site found at 1217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal SapI.rc site found at 2607


Design Notes

Starting from eucaryote DNA was a challenge to get a sequence without introns.


Source

The promoter was taken from the registry. 4CL and STS were amplified from grape cDNA.

References